Nitrate reductase of green algae is located in the pyrenoid.
نویسندگان
چکیده
Antibodies against nitrate reductase from Monoraphidium braunii have been used to determine the antigenic relationships of nitrate reductases from different green algae. Nitrate reductases from Chlamydomonas reinhardii, Chlorella fusca, Dunaliella salina, and Scenedesmus obliquus, were inhibited by, and cross-reacted with, antibodies raised against the enzyme from Monoraphidium braunii.These antibodies were also used to determine, by immunoelectron microscopy, the intracellular location of nitrate reductase in the aforementioned green algae. In all cases, the enzyme was specifically located in the pyrenoid.
منابع مشابه
Nitrate reductase activity: A solution to nitrate problems tested in free and Immobilized algal cells in presence of heavy metals
Every organism has different potential to accumulate NO3 from the environment. Nitrate reduction processes are perhaps most significant in maintaining water quality by alteration of nitrate to nitrite. A comparative study between the nitrate reductase NR activity of green and blue green algae in presence of heavy metals is being conducted to present a situation where nitrate reductase process m...
متن کاملExtracellular ferricyanide reduction and nitrate reductase activity in the green alga Monoraphidium braunii
The unicellular green alga, Monoraphidium braunii, reduced extracellular ferricyanide at high rates. Impermeability of the membranes to ferriand ferrocyanide was shown for untreated cells as for those permeabilized with 5% I-propanol. Fe starvation stimulated reduction rates by 50%, light or glucose were ineffective. Extracellular acidification largely corresponded to formation of the new anion...
متن کاملThe Presence of a Crystalline Matrix in Pyrenoids of the Diatom, Achnanthes Brevipes
It is generally known that the chloroplasts of most algae contain large bodies called pyrenoids around which form the photosynthetic reserves of the cell (for a general discussion, see reference 1). Most studies on the ultrastructure of algae have shown the pyrenoid to be composed of a finely granular, densely packed, homogeneous material (see references 2-6). Other studies have revealed a fibr...
متن کاملThe morphology of green hydra endosymbionts as influenced by host strain and host environment.
The ultrastructure of Chlorella-like algal endosymbionts from the Florida and English strains of green hydra was compared under different host feeding and photoperiodic regimes. Under standard conditions (host fed daily, 12-h photoperiod) the algae from the 2 strains exhibited considerable differences. The English symbionts had a pyrenoid, compact chloroplast membranes and vesiculated polyphosp...
متن کاملAction spectra for nitrate and nitrite assimilation in blue-green algae.
Action spectra for the assimilation of nitrate and nitrite have been obtained for several blue-green algae (cyanobacteria) with different accessory pigment composition. The action spectra for both nitrate and nitrite utilization by nitrate-grown Anacystis nidulans L-1402-1 cells exhibited a clear peak at about 620 nanometers, corresponding to photosystem II (PSII) C-phycocyanin absorption, the ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Plant physiology
دوره 79 4 شماره
صفحات -
تاریخ انتشار 1985